Figure 3 | Scientific Reports

Figure 3

From: Development of a plasmid free CRISPR-Cas9 system for the genetic modification of Mucor circinelloides

Figure 3

Genome editing strategy designed to disrupt the hmgR2 gene of Mucor circinelloides by the CRISPR-Cas9 method and positions of the primers used in the study (A) and PCR analysis of the transformants using the H2cDNS1 and H2cDNS8 primers (Table 2) (B). TGG indicates the PAM sequence; arrows show the orientations of the primers. Panel B: M, GeneRuler 1 kb DNA ruler (Thermo Scientific); 1, MS12 strain; 2, MS12-hmgR2 + pyrG strain.

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