Figure 3

Molecular and biochemical analysis of OkHMGR gene and protein. (A) PCR amplification product (1.7Kb) amplified with gene specific primers. (M-1Kb ladder, OK-amplified product), (B) SDS-PAGE analysis of His-tagged recombinant OkHMGR (lane 1: protein molecular weight marker; lane 1–4 OkHMGR enzyme), (C) Substrate saturation curves for HMG-CoA and (D) Substrate saturation curves for NADPH.