Figure 4

3-D structure of nanocapsid T = 1, conjugated to AuNC (a) Surface rendering of 3D reconstructed volumetric map of nanocapsid bound to AuNC, depicting both the outer surface (left) and capsid interior (right). The shell, middle, and protrusion domains are radially colored with gray, dark blue, and cyan, respectively. Scale bar is 6.0 nm. (b) Non-orthogonal intensity slice analysis around 5-fold axis showing high intensity regions associated with AuNC. Overall diameter of each AuNC measured to be 2.5 nm and distance from bottom to top of 5-fold measured to be 3.3 nm. (c) Plane intensity analysis showing distinct high intensity regions with a total diameter of 5.9 nm and distance between each AuNC 2.9 nm. (d) Quantitative comparison of surface intensity around the 5-fold axis between nanocapsid with (left) and without AuNC (right). Nanocapsid-bound AuNC showing distinct peaks around the 5-fold, not observed in the no-AuNC map. (e) Quantitative 1D intensity histogram comparing nanocapsid-bound AuNC (gold line) with nanocapsid (gray). Intensity peak ~1.8 nm away from the center of the 5-fold, in good agreement with observed position of AuNC.