Figure 5

3D reconstruction of AuNC-bound nanocapsid based on relaxed symmetry TEM analysis. (a) 3D modeling of AuNC-bound nanocapsid with 5 AuNC clusters arranged around the 5-fold axis in a ring-shaped array. (b) Following relaxed symmetry 3D-refinement of AuNC-bound nanocapsid, 5-fold vertices show an uneven distribution of AuNC from the relaxed symmetry reconstruction. These vertices are colored based on slice intensity analysis with 5-fold vertices having between 0–5 intensities attributable to AuNCs. (c) Illustration of various scenarios based on relaxed symmetry TEM 3D reconstruction results. Radial cueing 120 Å away from the nanocapsid origin.