Figure 7
BDNF expression is reduced in the hippocampus DS patients. (A) RT-qPCR quantification of BDNF mRNA in the hippocampus of adult DS patients (open symbols: □ males; ○ females) in comparison to age/sex matched non-trisomic controls (solid symbols: ■ males; ● females). Group averages (±SEM) are reported by lines. (B) Top, representative immunoblot for BDNF in protein extracts from human post-mortem whole hippocampus (gender is indicated above as squares for males and circles for females). Bottom, quantification of BDNF (expressed as percentage of controls) showed decreased expression in the hippocampus of adult DS patients in comparison to age/sex matched non-trisomic controls. Actin was used an internal standard. (C) Scatterplot representation of BDNF protein and mRNA expression for each individual samples. *P < 0.05, **P < 0.01 unpaired Student’s t-test. Full-length blots are presented in Supplementary Fig. 7.
