Figure 2 | Scientific Reports

Figure 2

From: Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues

Figure 2

Cell viability of the bioinks. (A) Representative images of Live/Dead staining used to determine cell viability of CaCl2, CaSO4 and CaCO3 crosslinkers for both high (HMW) and low MW (LMW) cast alginate constructs at the previously determined optimum crosslinking ratio (25:9 and 4:3 respectively). (B) Quantitative analysis of the cell viability for all of the bioinks. All bioinks were prepared in DMEM. Error bars denote standard deviation, n = 5. (C) Representative image of Live/Dead staining of a 3D printed construct printed with HMW alginate crosslinked with CaSO4 at the optimum crosslinking ratio of 25:9. (D) Quantitative analysis of the cell viability for cast and 3D printed high MW alginate crosslinked with CaSO4. All bioinks were prepared in DMEM. Error bars denote standard deviation, n = 5.

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