Figure 3

Immune cell migration is triggered by soluble factors delivered by CFC tissue, but not IFC tissue. Monocytes (CD14+) and T cells (CD3+) were separated from human PBMC. Thirty thousand cells in diet-medium were seeded on the porous membrane of a chemotaxis system. CFC or IFC tissue conditioned diet-medium (CM) was placed in the lower well. After 3 h, the number of migrated monocytes (a) and T cells (b) was analyzed. Migrated cells were defined as cells in the lower well plus cells attached to the bottom side of the membrane. Pure diet-medium served as negative control (Neg. Ctrl.), to define the random cell migration without a chemotactic gradient. Data are shown as the mean + SEM (n = 5, 3 replicates each) and analyzed with one-way ANOVA (Kruskal-Wallis test) *p < 0.05, **p < 0.01, ***p < 0.001.