Figure 6

Both types of cryopreserved tissue upregulate common surface molecules on macrophages, but do not influence their polarization state. Macrophages were cultured on the intima surface of CFC or IFC treated aortic tissue. Cells cultured on tissue culture plastic (TCP) served as control. After 2 days, macrophages were harvested, stained with fluorochrome-labeled human specific antibodies and analyzed by flow cytometry. Representative histograms and quantitative analyses of the mean fluorescence intensity (MFI) are shown for the surface markers CD16 (a) and CD14 (b), the M1 polarization marker CD80 (c) and HLA-DR (d) and the M2 polarization markers CD206 (e) and CD163 (f). The means of the data are shown (n = 11) and analyzed with one-way ANOVA (Kruskal-Wallis test) *p < 0.05, ***p < 0.01.