Figure 1
Concentration- and time-dependent EA-induced cell-death in SW982 cells. To examine whether EA causes cell-death in SW982 cells, EA at a concentration range between 0.3 nM and 100 nM was applied for 24 (a) and 48 h (b) and the cell-viability was assessed with WST-1 assay (three independent experiments). As comparisons, the same concentrations of EA were applied to HaCaT, Caco2, A549, and PC3 cells for 24 h and 48 h. (c) SW983 cells were exposed to 30 nM EA for 2, 6, 24, and 48 h and the cell-viability was assayed at each time (four independent experiments). (d) Effects of a putative TRPC4 inhibitor ML204 on EA-induced cell-death in SW982 cells. EA at 10 nM was applied for 48 h to SW982 cells pretreated with or without 50 μM ML204 (four independent experiments). Pooled data were averaged and expressed as mean ± SEM. The data were analyzed using one-way ANOVA (c) and Tukey’s test (d). **p < 0.01 compared with each group (d).
