Figure 2

Expression of TRPC subfamily TRPC1, TRPC4, and TRPC5 in SW982 cells. The mRNA expression of TRPC1, TRPC4, and TRPC5 was determined in SW982 cells with RT-PCR (a) and quantitative PCR analysis (b). Pooled data were averaged and expressed as mean ± SEM (b). (c) The isoform expression level of TRPC4 (TRPC4α and TRPC4β) was also examined in SW982 cells with RT-PCR. Human TRPC4α protein has an insertion of 84 amino acids in the C-terminus of human TRPC4β protein. For the whole segment of gels ((a) and (c)) see the Supplementary Fig. 2.