Figure 4
Pharmacological properties of EA-induced Icat composed of homo- and heteromeric channel with TRPC1 and TRPC4. After HEK cells with TRPC4α (a,b and e,f) and TRPC4α/C1 (c,d and g,h) were exposed to 100 nM EA, ML204 (a,b and c,d) and Clm (e,f and g,h) at 5 and 50 μM were applied. Ramp waveform pulses from −110 to + 90 mV for 400 ms were applied every 5 s and the peak amplitude of Icat at −80 and +80 mV was plotted against time (a,c,e, and g). A typical I-V was exhibited before and after application of 100 nM EA in the presence and absence of ML204 and Clm under whole-cell conditions (b,d,f and h). (i) The peak amplitude of IEA at −80 and +80 mV in the presence of inhibitors was normalized to that in the absence under each recording condition. Pooled data were averaged and expressed as mean ± SEM. The data were analyzed using student’s t-test. *p < 0.05 and **p < 0.01 compared with homomeric TRPC4α. The number in parenthesis indicates the number of independent cells used.
