Figure 5
Biophysical and pharmacological properties of EA-induced Icat in SW982 cells. After SW982 cells were exposed to 100 nM EA, ML204 (a–d) and Clm (e,f) at 5 and 50 μM were applied. Ramp waveform pulses from −110 to + 90 mV for 400 ms were applied every 5 s and the change of peak amplitude of Icat at −80 and +80 mV was plotted against time (a, c, and e). A typical I-V was exhibited before and after application of 100 nM EA in the presence and absence of ML204 and Clm under whole-cell conditions (b, d, and f). (g) The peak amplitude of IEA at −80 and +80 mV in the presence of inhibitors was normalized to that in the absence. Pooled data were averaged and expressed as mean ± SEM. The data were analyzed using Tukey’s test. **p < 0.01 and ##p < 0.01 compared with data without inhibitors and with 5 μM inhibitors, respectively. The number in parenthesis indicates the number of independent cells used.
