Figure 6
EA-induced Icat in SW982 cells depleted of TRPC1. EA at 100 nM was applied to SW982 cells pretreated with sc-RNA and si-TRPC1 to induce Icat. Ramp waveform pulses from −110 to + 90 mV for 400 ms were applied every 5 s. A typical I-V was exhibited in the presence and absence of EA in a SW982 cell pretreated with sc-RNA (a) and si-TRPC1 (b). The peak amplitude of EA-induced Icat (IEA) at −80 and +80 mV was summarized in SW982 cells pretreated with sc-RNA and si-TRPC1 (c). The relative amplitude of IEA at +80 to IEA at −80 mV was summarized as a ratio in SW982 cells pretreated with sc-RNA and si-TRPC (d). (e–i) After SW982 cells pretreated with sc-RNA and si-TRPC1 were exposed to EA, ML204 (5 and 50 μM) was applied and the change of peak amplitude of Icat at −80 and + 80 mV was plotted against time (e and g). A typical I-V was exhibited before and after application of EA in the presence and absence of ML204 in a SW982 cell pretreated with sc-RNA and si-TRPC (f and h). (i) The peak amplitude of IEA at −80 and +80 mV in the presence of ML204 was normalized to that in the absence. Pooled data were averaged and expressed as mean ± SEM. The data were analyzed using student’s t-test. **p < 0.01 compared with SW982 cells with sc-RNA. The number in parenthesis indicates the number of independent cells used.
