Table 3 Distribution of polymorphic alleles of non-synonymous mutations in the MdαE7.

From: Multiple mutations and overexpression of the MdaE7 carboxylesterase gene associated with male-linked malathion resistance in housefly, Musca domestica (Diptera: Muscidae)

			Population
			MSS−♀	MSS−♂	N-MRS- ♀	N-MRS− ♂	BC− ♀	BC− ♂	F2− ♀^b	F2− ♂^b
Frequency of Polymorphisms of Amino Acid Mutation Sites (%, ± SE)^a	S250T [TCA → ACA]	T/T	97.5 ± 2.5	1.25 ± 1.25	0	0	100	1.25 ± 1.25	58.14 ± 1.76	0
		T/A	2.5 ± 2.5	98.75 ± 1.25	0	6.25 ± 3.75	0	98.75 ± 1.25	41.86 ± 1.76	51.72 ± 2.68
		A/A	0	0	100	93.75 ± 3.75	0	0	0	48.28 ± 2.68
	W251S [TGG → TCG]	G/G	98.75 ± 1.25	2.5 ± 1.44	0	0	100	1.25 ± 1.25	58.18 ± 2.05	0
		G/C	1.25 ± 1.25	97.5 ± 1.44	0	0	0	98.75 ± 1.25	41.82 ± 2.05	50.99 ± 2.60
		C/C	0	0	100	100	0	0	0	49.01 ± 2.60
	M303I [ATG → ATA]	G/G	100	20 ± 4.08	0	0	100	6.25 ± 3.75	58.46 ± 1.64	0
		G/A	0	80 ± 4.08	8.75 ± 4.27	5 ± 2.89	0	93.75 ± 3.75	41.54 ± 1.64	50.99 ± 2.60
		A/A	0	0	91.25 ± 4.27	95 ± 2.89	0	0	0	49.01 ± 2.60
	L354F [CTT → TTT]	C/C	98.75 ± 1.25	12.5 ± 3.23	0	0	100	3.75 ± 3.75	58.50 ± 1.88	0
		C/T	1.25 ± 1.25	87.5 ± 3.23	11.25 ± 2.39	5 ± 2.89	0	96.25 ± 3.75	41.50 ± 1.88	50.99 ± 2.60
		T/T	0	0	88.75 ± 2.39	95 ± 2.89	0	0	0	49.01 ± 2.60
	S357L [TCA → TTA]	C/C	100	12.5 ± 4.79	0	0	100	11.25 ± 7.18	59.55 ± 2.14	0
		C/T	0	87.5 ± 4.79	13.75 ± 2.39	6.25 ± 3.75	0	88.75 ± 7.18	40.45 ± 2.14	51.72 ± 2.68
		T/T	0	0	86.25 ± 2.39	93.75 ± 3.75	0	0	0	48.28 ± 2.68
	W378R [TGG → CGG]	T/T	96.25 ± 2.39	1.25 ± 1.25	0	0	100	0	56.72 ± 1.71	0
		T/C	3.75 ± 2.39	98.75 ± 1.25	3.75 ± 2.39	2.5 ± 1.44	0	100	43.28 ± 1.71	50.99 ± 2.60
		C/C	0	0	96.25 ± 2.39	97.5 ± 1.44	0	0	0	49.01 ± 2.60
		G/G	96.25 ± 2.39	1.25 ± 1.25	0	0	100	0	58.14 ± 1.76	0
	S383T [AGT → ACA]	G/C	3.75 ± 2.39	98.75 ± 1.25	3.75 ± 2.39	5 ± 2.89	0	100	41.86 ± 1.76	50.99 ± 2.60
		C/C	0	0	96.25 ± 2.39	95 ± 2.89	0	0	0	49.01 ± 2.60
		T/T	100	7.5 ± 3.23	0	0	100	6.25 ± 3.75	58.83 ± 1.75	0
		T/A	0	92.5 ± 3.23	3.75 ± 2.39	2.5 ± 1.44	0	93.75 ± 3.75	41.17 ± 1.75	50.99 ± 2.60
		A/A	0	0	96.25 ± 2.39	97.5 ± 1.44	0	0	0	49.01 ± 2.60

^aValues represent mean ± SE for four replications of frequency (%) analyses of each mutation; the nucleotide polymorphisms are underlined. ^bData was synthesized from the four malathion-treated groups in corresponding gender.

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Table 3 Distribution of polymorphic alleles of non-synonymous mutations in the MdαE7.

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