Figure 3
From: E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface
Galectin-7 directly interacts with the extracellular domain of E-cadherin independently of glycosylation motifs. (a) In vitro binding assays were performed using recombinant wild type human galectin-7 (rGal7); CRD mutated human galectin-7 (R74S) and extracellular domain of mouse E-cadherin fused to human IgG1 Fc fragment (E-cad-Fc). Both WT and mutated galectin-7 (rGal7 and R74S) were precipitated with E-cad-Fc. (b) Immunoblot showing co-immunoprecipitations of galectin-7 performed on cell lysates in the presence of high sugar concentrations. (c) As in absence of competitive carbohydrate or in the presence of 15 mM sucrose, addition of 15 mM LacNAc did not prevent galectin-7 to immunoprecipitate E-cadherin. (d) Immunoprecipitations of galectin-7 were done after deglycosylation of protein extracts in non-denaturing conditions. Representative western blot data are shown in this figure.
