Figure 6
From: E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface
Association of galectin-7 with E-cadherin at the plasma membrane influences E-cadherin mediated adhesion strength. (a) Immunofluorescence images showing accumulation of rGal7-Cy3 (magenta) at the plasma membrane with E-cadherin (green) 30 min or 2 h after addition of 0.5 mg.ml−1 rGal7. Scale bar = 20 μm. (b) rGal-Cy3 enrichment at the plasma membrane was removed by 200 mM lactose washes. Scale bar = 20 μm. (c) Washing with lactose gave similar PLA staining than no washing for E-cadherin-Galectin-7 proteins. S100A1 – Galectin-7 staining served as a negative control. Scale bar = 15 μm. (d) rGal7-Cy3 (magenta) did not show important colocalisation with endocytosed E-cadherin (green). HECD-1 antibody was incubated 30 min à 37 °C to label endocytosed E-cadherin. Scale bar = 20 μm. (e) Schematic representation of the experimental design illustrating E-cadherin coated bead bound to HaCaT cells. Rapprochement of the magnetic tweezers will cause bead detachment. (f) Measurements of the distance required to detached E-cadherin coated beads from cells. Mean values ± s.e.m are represented.
