Figure 4 | Scientific Reports

Figure 4

From: lncTCF7 is a negative prognostic factor, and knockdown of lncTCF7 inhibits migration, proliferation and tumorigenicity in glioma

Figure 4

Knockdown of lncTCF7 inhibits glioma cell growth and results in the G0/G1 cell cycle G1 arrest. U87 and U251 cells were transfected with lncTCF7 shRNA, and the proliferation were tested by CCK-8 assay (A) and colony formation assay (B,C). U87 and U251 cells were transiently transfected with lncTCF7 shRNAs or NC shRNAs for 48 h, and the cell cycle profiles were examined by flow cytometry. (D) Knockdown of lncTCF7 inhibited TCF7, Sox2 and CyclinD1 expression through hindering Wnt signalling. Full-length images of cropped gels from Figs 3D and 4D are shown in Supplementary Information file. (E) Knockdown of lncTCF7 inhibited sphere formation in glioma stem cells. The right panel presents the statistical results as mean as the means ± SD. Scale bar, 500 μm.

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