Figure 4 | Scientific Reports

Figure 4

From: Transplantation of a quaternary structure neutralizing antibody epitope from dengue virus serotype 3 into serotype 4

Figure 4

Neutralization and binding of DENV4-specific and cross-reactive monoclonal antibodies on rDENV4/3 panel (A) ELISA binding curves of DENV3, DENV4, DENV4/3 M12, DENV4/3 M14 and DENV4/3 M16 by DENV4-specific mAbs show loss of 126, 131 DENV4 epitopes in the chimeric viruses but not the 5H2 DENV4 epitope. (B) U937-DC-SIGN Neutralization assays with these same DENV4-specific mAbs also show loss of 126, 131 DENV4 epitopes while 5H2 was retained. (C) ELISA binding curves with cross-reactive mAbs that are distal to the 5J7 epitope show similar binding to all viruses. Error bars represent the range of values. Graphs show one representative experiment of at least 2 independent experiments.

Back to article page