Figure 6 | Scientific Reports

Figure 6

From: Connecting Neuronal Cell Protective Pathways and Drug Combinations in a Huntington’s Disease Model through the Application of Quantitative Systems Pharmacology

Figure 6The alternative text for this image may have been generated using AI.

Protective compounds can induce cAMP. cAMP levels were determined in STHdh Q111 cells after incubation with benztropine (25 μM), domperidone (6 μM), isoetarine (50 μM), loxapine (6 μM), mianserin (25 μM), papaverine (25 μM), and sodium nitroprusside (66 μM) for 15, 30, and 120 minutes. Though isoetarine was the only compound to show a statistically significant change at 15 and 30 minutes, except for mianserin, the other compounds showed at least a two-fold increase in cAMP levels at 15 mins. Over time the induced levels of cAMP decreased back to the control levels. Forskolin significantly induced cAMP levels at 15 and 30 minutes with the highest levels seen at 15 minutes. The values are the average from three independent experiments (+/−S.E.) except papaverine where n = 2. All compounds except forskolin are plotted on the blue scale on the left, while forskolin is plotted on the grey scale on the right. The three panel rows are 15, 30, and 120 minutes. T-test was used to assess changes in cAMP levels relative to the STHdh Q111 cells treated with DMSO.

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