Figure 2

Distribution of AAV vectors in DRG. GFP-tagged AAV vectors (AAV2, 5, 6, 8, 9, and rh10) were placed intrathecally between the L4/5 vertebrae of immunodeficient mice by percutaneous lumbar puncture. At 4 weeks, DRGs were collected for immunofluorescence analyses for GFP positivity to determine AAV distribution. (A) Representative image of L3 DRGs obtained from AAV injected mice. Magnification, × 20. Scale Bar = 100 µm. (B) Quantifications of (A). (C,D) Quantifications of GFP positivity of (C) L2 and (D) L4 DRGs obtained from AAV injected mice. Presented as mean ± standard deviation, Significant differences were determined by one-way ANOVA with Tukey’s multiple comparisons. *p ≤ 0.05, ***p ≤ 0.0001, ****p ≤ 0.00001.