Figure 4
From: Tumor necrosis factor-alpha regulates photoreceptor cell autophagy after retinal detachment
TNF-α inhibition influences LC3B activity after RD. (A–D) The levels of increased LC3B were prolonged and increased moderately at 1, 3 and 7 days after RD (C), compared with attached retinas (A) and detached retinas (B). (D) The quantitative data of the ROIs for LC3B expression after RD with TNF-α inhibition. Error bars represent SD. (E,F) The protein levels of LC3B analyzed by Western blot. With Infliximab treatment, the increased expression of LC3B-II and LC3B-II/LC3B-I was maintained through 7 days after RD, while the level of LC3B decreased to baseline at 7 days after RD without TNF-α inhibition. P-values were calculated using ANOVA analysis by SPSS20. The complete statistic results can be find in Supplementary Table 2 and Supplementary Table 3. All conditions were significantly different with *P < 0.05, **P < 0.01, and ***P < 0.005.
