Figure 3
From: Peroxiredoxin activity is a major landmark of male fertility
Effect of decreased PRDX activity on viability, intracellular ATP, reactive oxygen species (ROS), and lactate dehydrogenase (LDH) levels in mouse spermatozoa. (A) Changes in viability (%) of control and conoidin A-treated spermatozoa. (B) Differences in bioluminescence intensity (proportional to levels of intracellular ATP) between control and conoidin A-treated spermatozoa. (C) Differences in fluorescence intensity (proportional to intracellular ROS activity) between control and conoidin A-treated spermatozoa. (D) Differences in absorbance (proportional to levels of intracellular LDH) between positive control (PC), control and conoidin A-treated samples. Data are mean of three replicates ± SEM. Values with superscripts (a,b,c) are significantly different between control and treatment groups by one-way ANOVA (P < 0.05) with Tukey’s multiple comparison test.
