Figure 6
From: Peroxiredoxin activity is a major landmark of male fertility
Effect of decreased PRDX activity on PKA activity and tyrosine phosphorylation. (A) Density of PKA substrates in control and conoidin A-treated samples (blue bar: ~55 kDa, red bar: ~35 kDa, green bar: ~30 kDa, purple bar: ~21 kDa). (B) Phospho-PKA substrates were probed with an anti-phospho-PKA antibody; lane 1: Control, lane 2: 1 µM conoidin A, lane 3: 10 µM conoidin A, lane 4: 100 μM conoidin A (uncropped blots are presented in Supplementary Fig. 1). (C) Density of tyrosine-phosphorylated proteins in control and conoidin A-treated spermatozoa (blue bar: ~70 kDa, red bar: ~55 kDa). (D) Tyrosine-phosphorylated proteins were probed with an anti-phosphotyrosine antibody; lane 1: Control, lane 2: 1 µM conoidin A, lane 3: 10 µM conoidin A, lane 4: 100 μM conoidin A (uncropped blots are presented in Supplementary Fig. 1). Data represent mean ± SEM, n = 3. Values with superscripts (A, B, C, a, b, c, d, α, β, γ) are significantly different between control and conoidin A-treatment groups by one-way ANOVA (P < 0.05) with Tukey’s multiple comparison test.
