Figure 4

qPCR validation of miRNA expression in synovial tissue from male mice at 1 and 6 weeks post sham or DMM surgery was determined. Data shown as expression relative to the average expression of two housekeepers (HK), U6 and 5s. In all cases there was no significant differences between DMM and sham within each time point. Each symbol represents an individual mouse (average of 2 technical replicates). Horizontal bars show the mean expression. Significant differences as determined by unpaired Mann-Whitney U test between groups connected by lines with exact p-values for each comparison indicated over the line. S1 = 1 week Sham; D1 = 1 week DMM; S6 = 6 weeks Sham; D6 = 6 weeks DMM.