Figure 5

Immunofluorescence staining for tyrosine nitration of IRβ in lung tissue of lean and high-fat diet-fed mice, treated or not with resveratrol (Resv; 100 mg/kg/day, 2 weeks). Representative immunostained DAPI (blue light), 3-NT (FITC green light) and IRβ (rhodamine red)-conjugated secondary antibody with the two color images merged to demonstrate co-localization of tyrosine nitration of IRβ (200× magnification).