Figure 5

Impaired B16F10 tumor growth and angiogenesis in Vcan ^hdf/+ mice. (A) Western blotting of tumors obtained from wild-type (WT) and Vcan ^hdf/+ mice consistently showing reduced versican (using anti-GAGβ) in the latter. Originally, the Western was performed on a single membrane and the extra lane in the middle was excised. (B) Comparison of tumor growth rate in wild-type and Vcan ^hdf/+ mice. Asterisks indicate the times after injection when tumor volume was statistically significant. n = 15 for each experiment. (C) Dot plots reporting tumor volumes in wild-type and Vcan ^hdf/+ mice. (D) Immunostaining of the tumor interior with anti-endomucin antibody (red) to identify capillaries (arrows). Sections were counterstained with hematoxylin. (E) Vessel counts obtained from wild-type and Vcan ^hdf/+ mice. n = 10 for each experiment. (F) Immunostaining of tumor vasculature using anti-endomucin (red). Nuclei were stained blue using Hoechst dye. White dashed lines Vcan ^hdf/+ timors indicates an abrupt boundary restricting most vasculature to the periphery (P); C, center.