Table 2 Specific primers used for amplification of the target genes of K. pneumoniae.
Target | Primer | Sequence (5′–3′) | Product size (bp) | Reference |
|---|---|---|---|---|
Capsular type K1 | MagAF1 | GGTGCTCTTTACATCATTGC | 1283 | |
MagAR1 | GCAATGGCCATTTGCGTTAG | |||
Capsular type K2 | K2wzyF1 | GACCCGATATTCATACTTGACAGAG | 641 | |
K2wzyR1 | CCTGAAGTAAAATCGTAAATAGATGGC | |||
Capsular type K5 | K5wzxF | TGGTAGTGATGCTCGCGA | 280 | |
K5wzxR | CCTGAACCCACCCCAATC | |||
Capsular type K20 | wzyK20F | CGGTGCTACAGTGCATCATT | 741 | |
wzyK20R | GTTATACGATGCTCAGTCGC | |||
Capsular type K54 | wzxK54F | CATTAGCTCAGTGGTTGGCT | 881 | |
wzxK54R | GCTTGACAAACACCATAGCAG | |||
Capsular type K57 | wzyK57F | CTCAGGGCTAGAAGTGTCAT | 1037 | |
wzyK57R | CACTAACCCAGAAAGTCGAG | |||
rmpA | rmpAF | ACTGGGCTACCTCTGCTTCA | 536 | |
rmpAR | CTTGCATGAGCCATCTTTCA | |||
Aerobactin | aerobactinF | GCATAGGCGGATACGAACAT | 556 | |
aerobactinR | CACAGGGCAATTGCTTACCT | |||
Alls | allsF | CCGAAACATTACGCACCTTT | 1090 | |
allsR | ATCACGAAGAGCCAGGTCAC | |||
kfuBC | kfuBC-F | GAAGTGACGCTGTTTCTGGC | 797 | |
kfuBC-R | TTTCGTGTGGCCAGTGACTC | |||
wcaG | wcaG-F | GGTTGGKTCAGCAATCGTA | 169 | |
wcaG-R | ACTATTCCGCCAACTTTTGC | |||
IucB | iucB-F | ATGTCTAAGGCAAACATCGT | 948 | |
iucB-R | TTACAGACCGACCTCCGTGA | |||
iroNB | iroNB-F | GGCTACTGATACTTGACTATTC | 992 | |
iroNB-R | CAGGATACAATAGCCCATAG | |||
ureA | ureA-F | GCTGACTTAAGAGAACGTTATG | 337 | |
urea-R | GATCATGGCGCTACCT(C/T)A | |||
wabG | wabG-F | CGGACTGGCAGATCCATATC | 683 | |
wabG-R | ACCATCGGCCATTTGATAGA | |||
uge | uge-F | GATCATCCGGTCTCCCTGTA | 535 | |
uge-R | TCTTCACGCCTTCCTTCACT | |||
fim | fim-F | TGCTGCTGGGCTGGTCGATG | 550 | |
fim-R | GGGAGGGTGACGGTGACATC | |||
ybtA | ybtA-F | ATGACGGAGTCACCGCAAAC | 960 | |
ybtA-R | TTACATCACGCGTTTAAAGG |
