Figure 3 | Scientific Reports

Figure 3

From: Transcriptomic signatures of cellular and humoral immune responses in older adults after seasonal influenza vaccination identified by data-driven clustering

Figure 3

Gene clusters (transcriptomic data 28 days post-vaccination) correlate meaningfully with immune response outcomes after vaccination. Gene expression (mRNA-Seq) data from PBMC samples of 138 older adults after influenza vaccination was clustered using WGCNA. Cluster membership is indicated by color. Left panel: heatmap of the correlation between each gene (row) and subjects’ immune response outcomes – cytokine recall responses (IFN-γ, IL-2, IL-4, IL-6, IL-10, TNF-α), H1N1 antibody responses (HAI/VNA), and memory B-cell responses (B-cell ELISPOT). Center column: cluster enrichment labels from Table 1. Right panel: Pearson’s correlation coefficient and p-value for relationships between cluster eigenvector (representing overall behavior) and subject immune outcomes. Heatmap shading indicates strength of correlations in both panels; see scale bar at right.

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