Figure 4

2-hydroxyisobutyrylated enzymes involved in TCA cycle, pentose phosphate pathway, pyruvate metabolism, glycolysis/gluconeogenesis and starch biosynthesis pathways. Proteins highlighted in green color are modified by 2-hydroxyisobutyrylation only, and proteins highlighted in red color are modified by both acetylation and 2-hydroxyisobutyrylation. The identified 2-hydroxyisobutyrylation sites and acetylation sites of enzymes in Fig. 4 are listed in Supplementary Table S3.