Figure 3 | Scientific Reports

Figure 3

From: Cannabinoid type 2 receptors in dopamine neurons inhibits psychomotor behaviors, alters anxiety, depression and alcohol preference

Figure 3

Tyrosine hydroxylase (TH) immunofluorescence staining of the VTA and SN of DAT-Cnr2 cKO mice after CMS for seven weeks. (a–d). Tyrosine hydroxylase (TH) immunofluorescence staining in the VTA and SN of non-stressed DAT-Cnr2 cKO (a,b) and VTA and SN of stressed DAT-Cnr2 cKO (c,d) mice. Microphotographs showing normal anatomical organization and distribution of the total population of dopaminergic neurons of the VTA (a) and SN (b) of control DAT-Cnr2 cKO mice and of the VTA (c) and SN (d) of DAT-Cnr2 cKO mice exposed to CMS and TH immunofluorescence staining. The morphology and size of individual TH-positive neurons, observed at a higher magnification, also showed a normal appearance in the two studied areas (inserts). Scale bar, 100 µm. This is supportive of the clear detection of CB2Rs in DA neurons in the VTA of wild type and absent in the DAT-Cnr2 cKO mice in the DA neurons with TH as marker.

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