Figure 7

CB2Rs mediated behaviors in the tetrad test. (a–d). The effects of selected CB1R, CB2R and mixed CB1R and CB2R ligands, WIN 55212-2 (3.0 mg/kg), ACEA (1.0 mg/kg) and JWH133 (20.0 mg/kg) decreased P < 0.05, locomotor activity (a) of the DAT-Cnr2 cKO and wild type mice in comparison to the vehicle treated mice of each genotype. In the tail flick test (b) at the doses used, WIN and ACEA but not the JWH compound produced analgesia in the wild type mice whereas in the DAT-Cnr2 cKO mice only the WIN compound induced analgesia (P < 0.05). In the hot plate test (e) WIN was potent in the induction of analgesia in both the wild type and DAT-Cnr2 cKO mice. ACEA and JWH were ineffective in the induction of analgesia in the wild type mice but JWH and not ACEA produced analgesia in the DAT-Cnr2 cKO mice. In the catalepsy test (c) the treatment with WIN, ACEA and JWH all induced catalepsy (P < 0.05) at the doses used with the DAT-Cnr2 cKO mice more sensitive than the wild type mice. Rectal temperature (d) was reduced by WIN and ACEA (P < 0.05) but not JWH at the selected doses used. Values are presented as mean ± SEM with 10–12 mice per group and *P < 0.05 for DAT-Cnr2 cKO and wild type mice and their respective vehicle treated controls.