Figure 2

Response of Gsto1 ^−/− mice to LPS. (a) Kaplan-Meyer plot showing survival of male mice treated with 10 mg/kg LPS (*p < 0.05, **p,0.01). (b) Body temperature of mice treated with LPS, Mean ± SE Gsto1 ^+/+ n = 7, Gsto1 ^+/− n = 6, Gsto1 ^−/− n = 5 (*p < 0.05, **p,0.01, ***p < 0.001).The significance of different comparisons are indicated as (* −/− vs +/+); († −/− vs +/−); (# +/− vs +/+). The dashed lines indicate times when there were too few surviving wild type mice to allow statistical comparison. Mice were ethically euthanazed if the decrease in temperature exceeded 10 °C or they reached a predetermined morbidity score. This experiment is representative of several experiments undertaken with different LPS concentrations (see Supplementary Fig. S1 for female mice). (c) liver of wild-type mouse treated with 50 mg/kg LPS for 3 hours demonstrating multiple foci of acute inflammation with necrosis (c) yellow arrows) which was absent in the liver of Gsto1 ^−/− mice receiving the same LPS treatment (d). (e) spleen histology of wild-type mouse treated with 50 mg/kg LPS for 6 hours demonstrating expansion of the white pulp due to accumulation of tingible body macrophages (yellow arrows) and increased inflammatory cells in the red pulp (green arrow), insert shows tingible body macrophages, ×630. (f) spleen of wild-type mouse treated with 50 mg/kg LPS for 6 hours with normal appearing red and white pulp.