Figure 4

Combination of bacterial CDAs during N-acetylation. (A) Base peak chromatogram of HILIC-ESI-MS analysis showing products after N-acetylation of the chitosan tetramer (D4, [GlcN]4, open circles) by two bacterial CDAs (VcCDA, NodB) yielding in a first step the mono-acetylated paCOS by VcCDA, and in a second step the double-acetylated paCOS by NodB. (B) summarizes this production route.