Figure 6 | Scientific Reports

Figure 6

From: Enzymatic production of all fourteen partially acetylated chitosan tetramers using different chitin deacetylases acting in forward or reverse mode

Figure 6

Production routes of all possible chitin and chitosan tetramers using CDAs to specifically deacetylate or N-acetylate (pa)COS; 1 represents the use of NodB, 2 of VcCDA, 3 of PesCDA, 4 of CnCDA4, and 5 of PgtCDA. Direction of routes are represented by arrows (grey: deacetylation, black: N-acetylation) and oligomers that are weak in color are not producible using this particular technique (de- or N-acetylation) (A) Production route of ten out of the 14 possible partially acetylated chitosan tetramers using different CDAs for deacetylation of the fully acetylated chitin tetramer as a substrate. (B) Production route of ten out of the 14 possible partially acetylated chitosan tetramers using different CDAs for N-acetylation under acetate-enriched conditions of the fully deacetylated chitosan tetramer as a substrate. (C) Overlay of production routes shown in part A (deacetylation) and B (N-acetylation), showing that all possible 14 partially acetylated chitosan tetramers are producible from the homotetramers A4 and D4.

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