Figure 7 | Scientific Reports

Figure 7

From: Enzymatic production of all fourteen partially acetylated chitosan tetramers using different chitin deacetylases acting in forward or reverse mode

Figure 7

Chromatographic purification up to analytical grade of the (pa)COS DDAD. (A) Base peak chromatogram of HILIC-ESI-MS analysis showing both buffer components (I, II, III, IV) and (pa)COS available in the sample after enzymatic N-acetylation of the GlcN tetramer by PesCDA and CnCDA4 (A3D1, A2D2, A1D3. (B) Base peak chromatogram of HILIC-ESI-MS analysis showing both buffer components (II) and (pa)COS available in the sample after purification over HILIC, rotational vacuum concentration and lyophilization.

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