Table 2 Detection of Xylella fastidiosa from plant tissues collected at various distances from the inoculation points after needle-inoculation. Sampling was performed at 1, 3, 6, 9, 12 and 24 months post inoculation (mpi). Experiment A and B were performed in a quarantine-equipped glasshouse and in a screen net tunnel, respectively.

Cultivar	Leaf and stem tissues														Roots
	1 mpi		3 mpi					6 mpi		9 mpi			12 mpi	24 mpi^c	12 mpi^d	24 mpi^e
	IP^a	1st^b	IP	1st	2nd	3rd	4th	5th	6th	6th	7th	8th	AP^f	AP	12 mpi^d	24 mpi^e
Experiment (A)
CELLINA DI NARDO’	7/10	2/10	7/10	4/10	4/10	2/10	0/10	9/10	0/10	9/10	0/10	nt^g	9/10	0/1	3/3	9/10
CORATINA	4/10	0/10	4/10	0/10	nt	nt	nt	2/10	0/10	2/10	0/10	nt	4/10	1/6	0/3	0/10
FRANTOIO	5/10	0/10	5/10	2/10	0/10	nt	ni	3/10	0/10	3/10	1/10	0/10	7/10	0/3	1/3	1/10
LECCINO	5/10	0/10	5/10	5/10	0/10	nt	nt	3/10	0/10	3/10	1/10	0/10	8/10	0/2	1/3	6/10
Mock inoculated controls (3 for each cultivar)	0/12	0/12	0/12	0/12	nt	nt	0/12	0/12	nt	nt	nt	0/12	0/12	0/12	0/3	0/10
Cultivar	Leaf and stem tissues														Roots
	1 mpi		3 mpi			6 mpi		9 mpi			12 mpi		24 mpi ^c		12 mpi ^d	24 mpi ^e
	IP ^a	1st ^b	1st	2nd	3rd	2nd	3rd	4th	5th	6th	6th	AP ^f	AP		12 mpi ^d	24 mpi ^e
Experiment (B)
CELLINA DI NARDO’	7/10	4/10	4/10	1/10	0/10	3/10	3/10	3/10	3/10	0/10	3/10	0/10	4/7		0	3/10
CORATINA	6/10	0/10	0/10	0/10	nt	1/10	0/10	0/10	nt	nt	nt	0/10	0/7		0	0/10
FRANTOIO	5/10	0/10	0/10	0/10	nt	0/10	0/10	0/10	nt	nt	nt	0/10	0/7		0	0/10
LECCINO	6/10	0/10	1/10	0/10	nt	0/10	0/10	0/10	nt	nt	nt	0/10	1/7		0	0/10
Mock-inoculated controls (3 for each cultivar)	0/12	0/12	0/12	0/12	nt	nt	0/12	0/12	nt	nt	nt	0/12	0/7		0	0/10

^aIP indicates inoculation point.
^b1^st–8^th = indicates the node above the IP.
^cThe figure indicates the number of positive plants when re-tested at 24 mpi among those testing negative at 12 mpi.
^dNumber of positive samples out of three subjected to the diagnostic test.
^eCumulative number of positive samples at 24 mpi.
^fAP = apical portion of the inoculated shoots.
^gNt = not tested.

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