Figure 2 | Scientific Reports

Figure 2

From: Generation of therapeutic protein variants with the human serum albumin binding capacity via site-specific fatty acid conjugation

Figure 2

Site-specific incorporation of AzF into position 160 of Uox (a) Crystal structure of Uox (PDB ID: 1WS2) viewed by the PyMOL program. W160 and the active site are marked in blue and red, respectively. (b) MALDI-TOF spectra of Lys-C-digested fragments derived from the Uox-160AzF (top) and Uox-WT (bottom). (c) The fluorescence image of protein gel of Uox-160AzF and Uox-WT reacted with DBCO-Rho taken by Bio-Rad ChemiDoc™ XRS+ (excitation: 302 nm, emission: 510–610 nm).

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