Figure 5 | Scientific Reports

Figure 5

From: Generation of therapeutic protein variants with the human serum albumin binding capacity via site-specific fatty acid conjugation

Figure 5

Comparison of fluorescence dye-conjugation yields of Uox variants. (a) Fluorescence (Fluorescence Panel; excitation: 302 nm, emission: 510–610 nm) and Coomassie-stained (Coomassie Panel) images of protein gels of Uox variant samples reacted with DBCO-Rho (Uox-22AzF (lane 1), Uox-23AzF (lane 3), Uox-112AzF (lane 5), Uox-138AzF (lane 7), Uox-160AzF (lane 9), and Uox-243AzF (lane 11)) and reacted with DBCO-amine followed by reaction with DBCO-Rho (Uox-22AzF (lane 2), Uox-23AzF (lane 4), Uox-112AzF (lane 6), Uox-138AzF (lane 8), Uox-160AzF (lane 10), and Uox-243AzF (lane 12)). (b) Fluorescence (Fluorescence Panel) and Coomassie-stained (Coomassie Panel) images of the protein gel of unreacted Uox variant (Uox-112AzF (lane 1) and Uox-160AzF (lane 4)), Uox variant reacted with DBCO-Rho (Uox-112AzF (lane 2) and Uox-160AzF (lane 5), and Uox variant reacted with DBCO-amine followed by reaction with DBCO-Rho (Uox-112AzF (lane 3) and Uox-160AzF (lane 6)). M indicates a lane for molecular weight standards. The images were taken by Bio-Rad ChemiDoc™ XRS+. The band intensities were analyzed by Image Lab software provided by Bio-Rad. Relative intensity values indicate the band intensities of Uox-112AzF samples relative to those of Uox-160AzF samples.

Back to article page