Figure 2 | Scientific Reports

Figure 2

From: Runx2 mediated Induction of Novel Targets ST2 and Runx3 Leads to Cooperative Regulation of Hypertrophic Differentiation in ATDC5 Chondrocytes

Figure 2

Expression of ST2L and sST2 splice variants during ATDC5 differentiation course. (A) RT-PCR examination of endogenous expression of transmembrane and soluble ST2 isoforms in ATDC5 and MC3T3-E1 cells (full gel is shown in Supplemental Fig. 2). PCR products with size 526 bp for murine ST2L and 360 bp for murine sST2 were observed on 1% agarose gels. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal control. ATDC5 Cells were cultured in maintenance media for four days to reach confluence. Maintenance media were then changed to differentiation media and cells were incubated for the time course of 28 days. Total RNA isolated from cells at day 0, 7, 14, 21 and 28 was assessed by q-PCR for expression levels of Col II (B), Col X (C), MMP-13 (D), ST2L (E), sST2 (F) and Runx2 (G). The sST2 mRNA expression level is calculated using ST2/ST2L ratio. The expression level of each gene was normalized to 18 s rRNA and represented as the mean ± S.D. n = 5. Statistical significance are *P ≤ 0.05, **P < 0.01, ***P < 0.001.

Back to article page