Figure 5 | Scientific Reports

Figure 5

From: Runx2 mediated Induction of Novel Targets ST2 and Runx3 Leads to Cooperative Regulation of Hypertrophic Differentiation in ATDC5 Chondrocytes

Figure 5

Distal and proximal promoter usage in ATDC5 and PHCs. (A) Schematic representation of 5′ promoter region of ST2 gene depicted up to exon2 (E2). Distal and proximal promoters are followed by non-coding E1a and E1b respectively. (B) Promoter usage analysis in ATDC5 chondrocytes. The ATDC5 stably transfected with empty vector (pCMV6) or mouse Runx2 cDNA vector (pCMV6-Runx2) were subjected to RT-PCR. The PCR product lengths representing ST2L and sST2 transcription from distal promoter are 1827 and 1718 bp respectively and transcription from proximal promoter generates 1863 bp fragment for ST2L and 1754 bp fragment for sST210. (C) Promoter usage analysis exhibited E1b associated expression of 1237 bp fragment sST2 isoform in 3 different PHCs8.

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