Figure 1
From: BKIP-1, an auxiliary subunit critical to SLO-1 function, inhibits SLO-2 potassium channel in vivo
Effects of BKIP-1 on the amplitude, voltage dependence, and activation rate of SLO-2 whole-cell currents in body-wall muscle cells. (a) Representative current traces and current-voltage relationship of SLO-2 (A) (n = 9), SLO-2 (B) (n = 10), and SLO-2 + BKIP-1 (n = 7), which represent shk-1(ok1581);bkip-1(zw10), shk-1(ok1581);bkip-1(zw17), and shk-1(ok1581) strains, respectively. (b) Conductance (G) – voltage relationship of the currents fitted to a Boltzmann function, and comparison of the G max and V 50 from the Boltzmann fit. (c) Sample current traces of the initial 500 ms in response to a +60-mV voltage step fitted to two exponentials (τ1 and τ2), and comparison of the τ1 and τ2 values among the three groups. (d) BKIP-1 has no effect on SHK-1 whole-cell currents in body-wall muscle cells. Shown are representative current traces, current-voltage relationship, and inactivation rate of whole-cell currents from SHK-1 (n = 18) and SHK-1 + BKIP-1 (n = 21), which represent slo-2(nf101);bkip-1(zw10) and slo-2(nf101) strains, respectively. Values are shown as mean ± SE. All statistical comparisons were made with two-way ANOVA except for G max and V 50 in panel b (one-way ANOVA). The asterisks indicate statistically significant differences between SLO-2 and SLO-2 + BKIP-1 (**p < 0.01, ***p < 0.001).
