Figure 8
From: BKIP-1, an auxiliary subunit critical to SLO-1 function, inhibits SLO-2 potassium channel in vivo
BKIP-1 interacts with SLO-2 in neurons. Bimolecular fluorescence complementation (BiFC) assays were performed by co-expressing SLO-2 and BKIP-1 tagged with the amino and carboxyl terminal portions of YFP (YFPa and YFPc), respectively. YFP signal was detected in motor neurons with full-length SLO-2 and BKIP-1 (top). Deletion of SLO-2 carboxyl terminal (middle) but not amino terminal (bottom) abolished the YFP signal. Shown are representative images of a segment of the ventral nerve cord. The bright signal at the top of each fluorescence image is from auto-fluorescence of the intestine. Scale bar, 20 μm.
