Figure 3 | Scientific Reports

Figure 3

From: Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken

Figure 3

Analysis of HA trimer content, thermal and acidic resistances of the TM-replaced recombinant H9N2-TM virus. Western-blot of H9N2-WT and H9N2-TM under non-reducing conditions. (B) Quantification of HA trimer content in H9N2-WT and H9N2-TM. **Indicates p < 0.01. (C) Fusion activity in erythrocytes of H9N2-TM and H9N2-WT at different pH values. *Indicates p < 0.05; **indicates p < 0.01. (D) Thermal resistance of H9N2-WT and H9N2-TM at a temperature ranging from 50 to 62 °C. Error bars represent the standard deviation from triplicate experiments. *Indicates p < 0.05; **indicates p < 0.01. (E) Acidic resistance of H9N2-WT and H9N2-TM in environments of pH 5.0, 5.2, and 7.4 at 37 °C using indirect immunofluorescene assay (IFA).

Back to article page