Figure 4
From: Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
Inactivation of NF-κB participated in H19-regulated cell growth. Sh-H19 transfected MM cells were treated with NF-κB inhibitor PDTC (50 μM for 24 h) compared with treatment with PBS as negative control. (A) Western blot was performed to determine phosphorylation changes of IκBα and P65. Cell proliferation (B), viability (C), and (D) colony formation decreased significantly upon PDTC stimulation. The synergistic inhibitory effects were induced in terms of IL-8 secretion by MM cells (E) and IL-6 secretion by hBMSC cells (F). (*P < 0.01, Figure is representative of 3 experiments with similar results).
