Figure 1
From: Transcriptomic analysis of mitochondrial TFAM depletion changing cell morphology and proliferation
Screening of DEGs related to the TFAM knockdown effects on the proliferation of MKN45 cells. (A) Left panel: western blot analysis showing the protein levels of siTFAM#1-, siTFAM#2-, and siCon-transfected MKN45 cells at 6 h, 24 h, and 48 h after transfection. Right panel: qPCR analysis of TFAM mRNA levels at 24 h after transfection (n = 3, mean ± SD). Student’s t test, ***P < 0.001. Full-length blots are presented in Supplementary Figure S1. (B) MTT assay of siTFAM#1-, siTFAM#2-, and siCon-transfected MKN45. A 570 nm was measured at 0, 24, 48, 72, and 96 h after transfection (n = 3, mean ± SD). Student’s t test, ***P < 0.001.
