Table 1 Top ten DEGs of TFAM knockdown.

From: Transcriptomic analysis of mitochondrial TFAM depletion changing cell morphology and proliferation

Gene

FC at 6 h

FC at 24 h

FC at 48 h

NUPR1

1.1

6.9*

16*

ITPK1

1.1

0.17*

0.23*

EFCAB12

1.2

5.3*

6.2*

PCK1

1.1

4.5*

12*

CFAP65

1.5

4.5*

6.1*

GLRX5

1.1

0.23*

0.21*

BAK1

0.87

0.32*

0.31*

MRPS33

0.93

0.32*

0.31*

NCAPG2

0.92

0.33*

0.32*

RAN

0.88

0.33*

0.42*

  1. The relative fold changes (FC) of mRNA levels after TFAM knockdown were calculated for 68 genes with their GAPDH-normalized ∆Cq values of qPCR. An average of ∆Cq values of the siTFAM#1 and siTFAM#2 cases in triplicate was divided by an average ∆Cq value of the siCon case in triplicate that were measured at 6, 24, and 48 h after siRNA transfection. The genes are listed according to decreasing order of the absolute values of fold changes at 24 h (Student’s t-test, *P < 0.05), and only the top ten genes other than TFAM are shown here.
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