Figure 3

The gut microbiota regulates intracellular vesicles and GLP-1 content in ileal Lpos cells. (a) Electron microscope images of ileal Lpos cells from GF and CONV-R GLU-Venus mice (n = 2–3). Red arrows indicate the densely packed vesicles and open arrows indicate the open (empty) type vesicles, scale 2 μm. (b) Intracellular GLP-1 content (normalized to total protein) in the lysate from primary crypt cultures of ileum of GF and CONV-R GLU-Venus mice (n = 3–4). Data are mean ± SEM. ***p < 0.001 indicates significance in CONV-R versus GF comparison.