Figure 3
From: Assembly of an atypical α-macroglobulin complex from Pseudomonas aeruginosa
Sedimentation velocity of MagD and Mag binary complexes (A) (Upper panel) Fitting of experimental sedimentation profiles of MagD using Sedfit and its residual (lower panel) (B) Results of the c(s) analysis for MagD (apoform), MagA:MagD (C), MagB:MagD (D), MagF:MagD (E) complexes. In (B), since the proportion of dimer does not increase with concentration, it probably does not result from a thermodynamic equilibrium. Samples were studied at 0.25, 0.5 and 1 mg/ml, at 20 °C with a rotor velocity of 35,000 rpm and an absorbance monitoring at 280 nm.
