Table 3 Data collection and structural parameters of the Mag complexes obtained using SAXS. Rh and Rg values obtained from SEC and DLS were also included for comparative purposes.
From: Assembly of an atypical α-macroglobulin complex from Pseudomonas aeruginosa
MagD | MagA:MagD | MagB:MagD | MagF:MagD | |
|---|---|---|---|---|
Data collection parameters | ||||
Instrument | LNLS-SAXS1 beamline | |||
Beam geometry | Pinhole geometry, Pilatus 300 k | |||
Wavelength (Å) | 1.544 | |||
s range (nm) | 0.068–2.8 | |||
Exposure time (sec) | 100 | |||
Temperature (°C) | 10 | |||
Concentration range (mg/mL) | 2–6 | |||
Structural parameters 1 | ||||
R g (nm) (from Guinier) | 5.0 ± 0.1 | 5. 8 ± 0.2 | 5.6 ± 0.1 | 5.3 ± 0.3 |
R g (nm) (from (P(r)) | 5.2 | 5.9 | 5.9 | 5.7 |
I(0) (cm−1) (from Guinier) | 0.0018 ± 3.8 10−6 | 0.00059 ± 3.1 10−6 | 0.0019 ± 5.4 10−6 | 0.0022 ± 6.4 10−6 |
Dmax (nm) | 17.6 | 20.1 | 19.8 | 19.3 |
Rh from SEC (nm) | 5.1 | 5. 3 | 5.2 | 5.2 |
Rh from DLS (nm); polydispersity (%) | 5.1 (7.4) | 5.3 (10.0) | 5.2 (9.7) | 5.2 (9.2) |
Software employed | ||||
Primary data reduction | FIT2d | |||
Data processing | PRIMUS/GNOM | |||
