Figure 1

Influence of Ca2+ (a) or Zn2+ (b) on the FSA activity. Enzyme activity was measured after pre-incubation the starch-free reaction mixtures with 0.5 mM Ca2+, 0.01 mM Zn2+, at 50 °C for 0 min and 30 min, respectively. For relative activity calculation, the activity of FSA in the absence of metal ions and without pre-incubation was set as 100%.